For example, plants and animals both have differentiated cells and tissues with specialized functions. In plants, only some tissues photosynthesize or absorb organic molecules; other tissues transport food and oxygen through the body, and still, others generate the molecular signals that govern development. In both plants and animals, only a small subset of all cells contributes to reproduction. Because of this functional differentiation, cell or tissue loss can be lethal for the entire organism.

There is one more feature of complex multicellular organisms that is key to understanding their biology: They have a three­ dimensional organization, so only some cells are in direct contact with the environment. Cells that are buried within tissues, relatively far from the exterior of the organism, do not have direct access to nutrients or oxygen. Therefore, interior cells cannot grow as fast as surface cells unless there is a way to transfer resources from one part of the body to another. Similarly, interior cells do not receive signals directly from the environment, even though all cells must be able to respond to environmental signals if the organism is to grow, reproduce, and survive. Complex multicellular organisms, therefore, require mechanisms for transferring environmental signals received by cells at the body’s surface to interior cells, where genes will be activated or repressed in response. Thus growth and development in complex multicellular organisms can be defined as increasing or decreasing gene expression in response to molecular signals from surrounding cells.

What are the characteristics that are found in both simple colonies of cells and complex multicellular organisms?
A) the ability of cells to communicate

So why is diffusion effective only over short distances? Diffusion is the random motion of molecules, with net movement from areas of higher to lower concentration. The distance and speed at which molecules can diffuse depends on several factors: (1) how great the concentration difference is, large larger the differences the faster the diffusion (2) distance between concentration differences, the closer the two concentrations the faster the rate of diffusion (3) the size of the molecule, smaller molecules tend to diffuse faster than larger ones, and (4) the nature of the medium the molecule is diffusing in; molecules diffuse faster if the medium is less thick (think air vs. water). Because diffusion supplies key molecules for metabolism, it exerts a strong constraint on the size, shape, and function of cells and ultimately how eukaryotic organisms function.

Let’s use oxygen as an example. Most eukaryotes require oxygen for respiration. If the cells within a tissue must rely on diffusion for their individual oxygen supply, the thickness of the tissue is limited by the concentration difference in oxygen between the cells in the tissue and the surrounding environment. The concentration difference between the two areas depends on the amount of oxygen in the environment and the rate at which oxygen is used for respiration inside the organism. The distance between the environment and the cell that is using oxygen also plays a role.

Which of these would increase the rate of diffusion of a molecule?
A) a high concentration gradient between two areas involved

The answer is that oxygen binds to molecules of hemoglobin in red blood cells and then is carried through the bloodstream to distant sites of respiration. We circumvent diffusion by actively pumping oxygen-rich blood through our bodies. Most invertebrate animals lack well-defined blood vessels instead they have other mechanisms that circulate fluids freely throughout the body cavity. Indeed, without a mechanism like bulk flow, animals could not have achieved the range of size, shape, and function familiar to us.

Complex organisms other than animals also rely on bulk flow. A redwood tree must transport water upward from its roots to leaves that may be 100 m above the soil. If plants relied on diffusion to transport water, they would be only a few millimeters tall. How, then, do they move water? Plants move water by bulk flow through a system of specialized tissues powered by the evaporation of water from leaf surfaces. Vascular plants also have specialized tissues for the transport of nutrients and signaling molecules upward and downward through roots, stems, and leaves.

Multicellular fungi transport nutrients through networks of filaments that may be meters long, relying on osmosis to pump materials from sites of absorption to sites of metabolism. The giant kelps have an internal network of tubular cells that transports molecules through a body that can be tens of meters long.  In general, when some cells within an organism are buried within tissues, far from the external environment, bulk flow is required to supply those cells with molecules needed for metabolism.

In addition to a means to move needed molecules over long distances and in sufficient concentration, there are three other general requirements for complex multicellular life: (1)Cells must stick together; (2) they must communicate with one another, and (3) they must participate in a network of genetic interactions that regulates cell division and differentiation. Once these are in place, the stage is set for the evolution of specialized tissues and organs in complex multicellular organisms.

If a fertilized egg is to develop into a complex multicellular organism, it must divide many times, and the cells produced from those divisions must stick together. In addition, they must retain a specific spatial relationship with one another in order for the developing organism to function. Most animal cells release materials into the extracellular space. The primary components of these materials are proteins, and the most abundant protein is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. Some cells attach themselves to this matrix by means of other transmembrane proteins called integrins and transmembrane proteins, especially cadherins, can also be involved in the formation of molecular attachments between cells. The extracellular matrix also allows the cells within the tissue to communicate with each other.

In plants, the cell wall connects cells together into tissues. The plant cell wall is composed predominately of cellulose fibers which are embedded in a matrix of pectin and hemicellulose. These molecules allow plant cells to attach to each other to create the complex tissue structures that support the overall plant structure. Fungi also have cell walls, although they are composed of chitin rather than cellulose, that are involved in connecting the cells together into multicellular structures.

In complex multicellular organisms, it is not sufficient for cells to adhere to one another. They must also be able to communicate. Communication is important during development, guiding the patterns of gene expression that differentiate cells, tissues, and organs. The functional integration of cells within tissues and tissues within organs also depends on the flow of information among cells. Cells communicate using signaling molecules (generally a protein) synthesized by one cell which binds with a receptor protein on the surface of a second cell. This interaction can essentially flip a molecular switch that activates or represses gene expression in the receptor cell’s nucleus or stimulates another response from the receiving cells.  Molecular evidence indicates that many of the signaling pathways used for communication between cells in complex multicellular organisms first evolved in single-celled eukaryotes.

What function might molecular signals and receptors have had in the ancestors of complex organisms?

All cells have transmembrane receptors that respond to signals from the environment. In some cases, the signal is a molecule released by a food organism (such as the bacteria that induce simple multicellularity in choanoflagellates), and in some cases the cells sense nutrients, temperature, or oxygen level. Single­ celled eukaryotes also communicate with other cells within the same species, for example, to ensure that two cells can find each other to fuse in sexual reproduction. Signaling between two cells within a complex multicellular organism (plant, animal, or fungal) can be seen as a variation on this more general theme of a cell responding to other cells and the physical environment.

While all eukaryotic cells have molecular mechanisms for communication between cells, complex multicellular organisms have distinct cellular pathways for the movement of molecules from one cell to another. For example, animals more complex than sponges have gap junctions, protein channels that allow ions and signaling molecules to move from one cell into another. Gap junctions not only help cells to communicate with their neighbors, they allow targeted communication between a cell and specific cells adjacent to it.

The innovation of complex multicellularity was to differentiate cells in space instead of time. In a three-dimensional multicellular organism, only surface cells are in direct contact with the outside environment. Interior cells are exposed to a different physical and chemical environment because nutrients, oxygen, and the light become less abundant with increasing depth within tissues. In effect, there is a gradient of environmental signals within multicellular organisms. We might, therefore, hypothesize that in the earliest organisms with three-dimensional multicellularity, a nutrient or oxygen gradient triggered oxygen- or nutrient-starved interior cells to differentiate, much as happens to trigger the formation of resting cells in their single-celled relatives. With increasing genetic control of cellular responses to signaling gradients, the seeds of complex development were sown.

Bulk flow, which transports nutrients, oxygen, and water within complex multicellular organisms, also carries developmental signals. Signals carried by bulk flow can travel far greater distances through the body than signals transmitted by diffusion alone. For example, in animals the endocrine system releases hormones directly into the bloodstream, enabling them to affect cells far from those within which they formed. Thus, the sex hormones estrogen and testosterone are synthesized in reproductive organs but regulate development throughout the body, contributing to the differences between males and females. In this way, signals carried by bulk flow can induce the formation of distinct cell types and tissues along the path of signal transport.

What is considered to be the greatest factor that leads to cell differentiation within complex multicellular organisms?
A) the lack of oxygen in internal spaces
B) the differing environments the cells are found in
C) the three-dimensional structure of the organism

2) In what order are the following thought to have evolved to have given rise to complex multicellularity?

3) How does cell differentiation differ between unicellular and complex multicellular organisms?A) Unicellular organisms do not exhibit cell differentiation
B) Multicellular organisms have a spatial differentiation of cells
C) Unicellular organisms have a spatial differentiation of cells

Animal cell connections:  A tight junction is a watertight seal between two adjacent animal cells. The cells are held tightly against each other by proteins (predominantly two proteins called claudins and occludins). This tight adherence prevents materials from leaking between the cells; tight junctions are typically found in epithelial tissues that line internal organs and cavities and comprise most of the skin. Also found only in animal cells are desmosomes, which act like spot welds between adjacent epithelial cells. Short proteins called cadherins in the plasma membrane connect to intermediate filaments to create desmosomes. The cadherins join two adjacent cells together and maintain the cells in a sheet-like formation in organs and tissues that stretch, like the skin, heart, and muscles. Gap junctions in animal cells are like plasmodesmata in plant cells in that they are channels between adjacent cells that allow for the transport of ions, nutrients, and other substances that enable cells to communicate. Structurally, however, gap junctions and plasmodesmata differ. Gap junctions develop when a set of six proteins (called connexins) in the plasma membrane arrange themselves in an elongated donut-like configuration called a connexon. When the pores (“doughnut holes”) of connexons in adjacent animal cells align, a channel between the two cells forms. Gap junctions are particularly important in cardiac muscle: The electrical signal for the muscle to contract is passed efficiently through gap junctions, allowing the heart muscle cells to contract in tandem.